First, the mechanism of ozone killing microorganisms

There are not many researches on the mechanism of action. According to foreign literature reports, the oxidative capacity of nascent oxygen produced by ozone decomposition destroys microbial DNA or RNA, and can also act on cell membranes, penetrate the cell wall and enter the cell wall, and act on the outer shell lipoprotein and The inner lipopolysaccharide changes the permeability of the cells and eventually causes the cells to dissolve and die.

Second, the mechanism of ozone air purification device disinfection

(1) Ozone production method

1. Photochemical ultraviolet method produces ozone high-ozone ultraviolet light - produces 253.7nm ultraviolet light while generating a large amount of ozone

2, along the discharge method plasma and ozone work together - air sterilizer

3, discharge method tubular ozone generator

(2) Killing of various microorganisms

1. Ozone is a broad-spectrum fungicide that kills bacterial propagules, cell spores, viruses, and fungi.

2. When there is sufficient humidity in the air, the ozone gas has a strong killing effect on the air and the surface microorganisms.

3. The bacteria liquid was sprayed in the air and ozone was used as a sterilization test. The results showed that the killing rate of ozone to the black spores in the aerosol was 5.67 mg/m 3 for 60 minutes, and the killing rate was over 99%; Staphylococcus aureus 0.21 mg/ The m 3 30-minute kill rate was 99.9%; when the ozone concentration reached 30 mg/m 3 for 15 minutes, the fungal kill rate was over 92%.

Third, the characteristics of ozone disinfection

? Ozone is a gas at normal temperature. After cold pressing, it can be a liquid. The solubility in water is 3%. At room temperature, it can liberate new oxygen.

Ozone gas is explosive, has a special odor, is toxic to human body, contains 0.02 - 0.04ppm in the air, and can be smelled. The maximum allowable concentration in the atmosphere is 0.2 mg / m 3 (about 0.1ppm)

Ozone has a strong oxidizing effect, which can kill various bacterial propagules. Different bacteria have different sensitivities to ozone. The ozone-sensitive bacteria include Bacillus subtilis and Staphylococcus aureus.

Concentration is an important factor in determining the disinfection effect of ozone. The degree of ozone killing of microorganisms is related to the concentration of ozone, but has little to do with contact time.

• Ozone against viruses and cell spores, when the ozone concentration does not reach the inactivated dose, the prolonged time does not increase the kill rate.

Fourth, factors affecting ozone sterilization

? The effect of ozone concentration: Concentration is an important factor in determining the effectiveness of ozone disinfection, and has little to do with contact time.

Impact of humidity: When ozone is used for air disinfection, the relative humidity is high, the sterilization effect is good, and the bacteria on the dry surface are difficult to kill.

? The impact of organic matter: can significantly reduce the killing effect of ozone on microorganisms, thus failing to achieve the desired disinfection effect.

? The effect of temperature: As the temperature increases, the bactericidal effect of ozone increases, but in actual use, the increase in temperature will affect the disinfection effect. (The solubility in water is reduced, and the degradation in the air is accelerated to reduce the ozone concentration)

Fifth, the application of ozone air disinfection machine

It can be used for air disinfection in the following spaces: operating room, sterile room, ward, medical and sanitary products manufacturer, hospital pediatric ward, gynecological examination room, injection room, dressing room, burn ward, etc. Ozone disinfection can be used for all kinds of general wards and rooms with a total number of bacteria ≤ 500.

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